中国水稻科学

中国水稻科学 ›› 2014, Vol. 28 ›› Issue (2): 148-154.DOI: 10.3969/j.issn.1001-7216.2014.02.006

• 研究报告 • 上一篇    下一篇

利用InDel和SSILP标记分析北方粳型超级稻的遗传组成

刘丹# ,王嘉宇# ,孙健,唐亮,陈温福*   

  1. 沈阳农业大学 水稻研究所/农业部东北水稻生物学与遗传育种重点实验室/北方超级粳稻育种教育部重点实验室, 沈阳 110866;
  • 收稿日期:2013-05-06 修回日期:2013-06-07 出版日期:2014-03-10 发布日期:2014-03-10
  • 通讯作者: 陈温福*
  • 基金资助:

    现代农业产业技术体系建设专项资金资助项目（CARS0113）； 辽宁省科技攻关重大项目（2012201002）； 国家科技支撑计划资助项目（2011BAD35B0201， 2011BAD16B14）。

Genetic Components Analysis on Super japonica Rice Using InDel and SSILP Molecular Markers

LIU Dan# , WANG Jiayu# , SUN Jian, TANG Liang, CHEN Wenfu*   

  1. Rice Research Institute, Shenyang Agricultural University /Key Laboratory of Northeast Rice Biology, Genetics and Breeding of Ministry of Agriculture/Key Laboratory of Northern japonica Super Rice Breeding, Ministry of Education, Shenyang 110161, China;
  • Received:2013-05-06 Revised:2013-06-07 Online:2014-03-10 Published:2014-03-10
  • Contact: CHEN Wenfu*

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摘要： 为明确籼稻血缘对北方粳型超级稻育种的贡献，利用89对籼粳特异性引物（34对InDel和55对SSILP）对15份粳型超级稻品种以及与其系谱密切相关的21份材料（12份1977-1999年育成的常规粳稻品种和9份1963-2000年育成的日本品种）的遗传组成进行分析。结果表明，15份粳型超级稻品种均含有一定的籼型血缘，且不同地区超级稻品种所含籼型基因频率（Fi）有所不同；对不同年代育成的参试材料的Fi进行比较分析，发现超级稻品种籼型基因型频率最高，Fi为0.068；12份常规粳稻品种次之，Fi为0.033；9份日本品种几乎不含有籼型血缘，Fi为0.011；表明随着时间的推进，近现代以来育成品种的籼型基因频率逐渐增加。

关键词: 粳型超级稻, 籼型血缘, 遗传组成, 籼粳特异性分子标记

Abstract: Genetic background of 15 superrice and their 21 pedigreerelative varieties (including  12 cultivars released from 1977 to 1999 in northeast and 9 japanese varieties bred from 1963 to 2000) were revealed based on 89 subspecificdifferentiation molecular markers (34 InDel and 55 SSILP). The results showed that indica linage have already introgressed into the genomes of 15 superrice varieties at different level which represented by indicatype frequency (Fi ). The Fi  variation of superrice varieties reflected registration periods difference, varieties bred in 2005-2011 were the  highest (Fi=0.068), followed by 1977-1999 (Fi=0.033) and rarely in 1963-2000 (Fi=0.011). Consequently, indicaallele frequencies of recent varieties were gradually increased. And this fact demonstrates that a certain proportion of indica linage introducing is an important strategy to the increased yield of northern japonica rice. 

Key words: super japonica rice, indica blood relationship, genetic components, subspecificdifferentiation molecular marker

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